Lab Members
Paraformaldehyde fix recipe Cryopreservation of tissue before cryosectioning Crysectioning Maceration of Aiptasia pallida host cells In situ hybridization protocol for tiny Aiptasia pallida Confocal microscopy of tiny Aiptasia pallida Fixation and embedding for EM immunocytochemistry
Host-free algal DNA extraction Algae-free host RNA extraction RNAeasy-Trizol hybrid RNA extraction protocol RNA gel Preparing an agarose gel for blotting Northern hybridization DIG High Prime DNA labeling and detection kit DIG wash and detection Primer design dsRNA synthesis and incubation for RNA interference Semi-quantitative PCR
Western Blotting Protein extraction of freshly isolated algae from A. elegantissima DAN assay for detection of NO in tissues
Daily cleaning of F. scutaria larval cultures Isolation of freshly isolated algae from adult corals Infection of F. scutaria larvae with freshly isolated algae Quantification of infection success in F. scutaria algae
Aiptasia husbandry Inoculation of tiny A. pallida with algae Heat shock and bleaching of A. pallida Cold shock and bleaching of A. pallida dsRNA synthesis and incubation for RNA interference Maceration of Aiptasia pallida host cells Confocal microscopy of tiny Aiptasia pallida DAN assay for detection of NO in tissues
Algal cultures Algal cultures upated 2008 Algal cell counts Isolation of freshly isolated algae from adult corals Inoculation of F. scutaria larvae with freshly isolated algae Quantification of infection success in F. scutaria algae Inoculation of tiny A. pallida with algae Host-free algal DNA extraction Protein extraction of freshly isolated algae from A. elegantissima
Anthopleura cold tank maintenance
Cold tank maintenance
